Glucokinase/GCK Protein, Human

Glucokinase/GCK proteins catalyze hexose phosphorylation, converting D-glucose, D-fructose, and D-mannose into their respective hexose 6-phosphates. Glucokinase/GCK Protein, Human is the recombinant human-derived Glucokinase/GCK protein, expressed by E. coli , with tag free.

The Glucokinase (GCK) protein is a pivotal player in glucose metabolism, catalyzing the phosphorylation of hexoses such as D-glucose, D-fructose, and D-mannose, to form hexose 6-phosphate—specifically, D-glucose 6-phosphate, D-fructose 6-phosphate, and D-mannose 6-phosphate. Notably, GCK exhibits a weaker affinity for D-glucose compared to other hexokinases and is most effective when glucose is abundant. Its predominant expression in pancreatic beta cells and the liver positions GCK as a rate-limiting step in glucose metabolism within these tissues. Acting as a glucose sensor in pancreatic beta cells, GCK's low glucose affinity allows it to modulate insulin secretion in coordination with glucose concentrations. In the liver, GCK plays a crucial role in facilitating glucose uptake and conversion, acting as an insulin-sensitive determinant of hepatic glucose usage. Additionally, GCK is essential for providing D-glucose 6-phosphate in the synthesis of glycogen and mediates the initial step of glycolysis by catalyzing the phosphorylation of D-glucose to D-glucose 6-phosphate.

Specific activity is 2022.238 pmol/min/µg. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH 8.0 at 37°C.

Human

E. coli

Tag Free

P35557-1/NP_000153.1 (L2-Q465)

2645  [NCBI]

LDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Approximately 55 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.