glycoprotein B/gB Protein, HHV-2 (Cell-Free, His)

Glycoprotein B (gB) is a key envelope glycoprotein that forms unique spikes on the surface of viral particles and plays a crucial role in the initial attachment of the heparan sulfate moiety of the host cell surface proteoglycan. This interaction is a fundamental step in the viral entry process. glycoprotein B/gB Protein, HHV-2 (Cell-Free, His) is the recombinant Virus-derived glycoprotein B/gB protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of glycoprotein B/gB Protein, HHV-2 (Cell-Free, His) is 882 a.a., with molecular weight of 99.7 kDa.

Glycoprotein B (gB) is a critical envelope glycoprotein integral to the structure of virions, forming spikes on the viral envelope surface. Its primary role lies in the initial attachment to heparan sulfate moieties present on host cell surface proteoglycans. This interaction is essential for the initial stages of viral entry into the host cell. Subsequently, gB is actively involved in the fusion of viral and cellular membranes, facilitating the entry of the virus into the host cell. Following initial binding to host receptors, the fusion process is orchestrated by the fusion machinery, consisting of at least gB and the heterodimer gH/gL. Additionally, gB may play a role in mediating the fusion between the virion envelope and the outer nuclear membrane during virion egress. Structurally, gB exists as a homotrimer, linked by disulfide bonds, and it interacts with heparan sulfate proteoglycans as well as the gH/gL heterodimer, highlighting its pivotal role in the complex mechanisms of viral entry and egress (

Virus

E. coli Cell-free

N-10*His

P08666 (A23-L904)

/

APAAPAAPRASGGVAATVAANGGPASRPPPVPSPATTKARKRKTKKPPKRPEATPPPDANATVAAGHATLRAHLREIKVENADAQFYVCPPPTGATVVQFEQPRRCPTRPEGQNYTEGIAVVFKENIAPYKFKATMYYKDVTVSQVWFGHRYSQFMGIFEDRAPVPFEEVIDKINTKGVCRSTAKYVRNNMETTAFHRDDHETDMELKPAKVATRTSRGWHTTDLKYNPSRVEAFHRYGTTVNCIVEEVDARSVYPYDEFVLATGDFVYMSPFYGYREGSHTEHTSYAADRFKQVDGFYARDLTTKARATSPTTRNLLTTPKFTVAWDWVPKRPAVCTMTKWQEVDEMLRAEYGGSFRFSSDAISTTFTTNLTEYSLSRVDLGDCIGRDAREAIDRMFARKYNATHIKVGQPQYYLATGGFLIAYQPLLSNTLAELYVREYMREQDRKPRNATPAPLREAPSANASVERIKTTSSIEFARLQFTYNHIQRHVNDMLGRIAVAWCELQNHELTLWNEARKLNPNAIASATVGRRVSARMLGDVMAVSTCVPVAPDNVIVQNSMRVSSRPGTCYSRPLVSFRYEDQGPLIEGQLGENNELRLTRDALEPCTVGHRRYFIFGGGYVYFEEYAYSHQLSRADVTTVSTFIDLNITMLEDHEFVPLEVYTRHEIKDSGLLDYTEVQRRNQLHDLRFADIDTVIRADANAAMFAGLCAFFEGMGDLGRAVGKVVMGVVGGVVSAVSGVSSFMSNPFGALAVGLLVLAGLVAAFFAFRYVLQLQRNPMKALYPLTTKELKTSDPGGVGGEGEEGAEGGGFDEAKLAEAREMIRYMALVSAMERTEHKARKKGTSALLSSKVTNMVLRKRNKARYSPLHNEDEAGDEDEL

99.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.