Glypican-1/GPC1 Protein, Mouse (HEK293, His)

Glypican-1 is a cell surface proteoglycan containing heparan sulfate that binds alpha-4 (V) collagen and aids in Schwann cell myelination. Glypican-1/GPC1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Glypican-1/GPC1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Glypican-1/GPC1 Protein, Mouse (HEK293, His) is 506 a.a., with molecular weight of ~60 kDa.

Glypican-1 (GPC1) Protein is a cell surface proteoglycan characterized by the presence of heparan sulfate. It binds to alpha-4 (V) collagen through its heparan sulfate side chains, playing a role in Schwann cell myelination. Additionally, GPC1 may act as a catalyst, enhancing the conversion rate of prion protein PRPN(C) to PRNP(Sc) by associating with both forms of PRPN through its heparan sulfate side chains, facilitating their interaction and targeting them to lipid rafts. GPC1 is essential for proper skeletal muscle differentiation, as it sequesters FGF2 in lipid rafts, preventing its binding to receptors (FGFRs) and inhibiting FGF-mediated signaling. Moreover, GPC1 exhibits the ability to bind Cu(2+) or Zn(2+) ions.

2 μg/mL (100 μL/well) of immobilized Human FGFb can bind Mouse GPC1-His with an ED₅₀ value of 0.4-2 μg/mL.

Mouse

HEK293

C-6*His

Q9QZF2 (D24-S529)

14733  [NCBI]

DPASKSRSCSEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTS

Approximately 60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.