Glypican-3/GPC3 Protein, Human (S495A, HEK293, Fc)

The Glypican-3 (GPC3) protein is a cell surface proteoglycan that plays complex regulatory roles in key signaling pathways critical to developmental processes. Through its GPI anchoring, GPC3 negatively regulates the Hedgehog signaling pathway by competing with the Hedgehog receptor PTC1 for binding to Hedgehog protein, leading to complex internalization and subsequent lysosomal degradation. Glypican-3/GPC3 Protein, Human (S495A, HEK293, Fc) is the recombinant human-derived Glypican-3/GPC3 protein, expressed by HEK293 , with C-mFc labeled tag.

GMP Glypican-3 (GPC3) Protein, a cell surface proteoglycan, orchestrates intricate regulatory roles in key signaling pathways crucial for developmental processes. Through its GPI-anchor, GPC3 negatively modulates the hedgehog signaling pathway by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins, leading to complex internalization and subsequent lysosomal degradation. Simultaneously, it exerts positive regulation on both canonical and non-canonical Wnt signaling pathways by binding to the Wnt receptor Frizzled, enhancing the interaction between Frizzled and Wnt ligands. GPC3 binds to CD81, reducing the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Additionally, GPC3 inhibits the dipeptidyl peptidase activity of DPP4. Functionally, GPC3 plays pivotal roles in limb patterning, skeletal development, renal branching morphogenesis, and coronary vascular development. It also modulates the effects of growth factors BMP2, BMP7, and FGF7 on renal branching morphogenesis and contributes to the regulation of cell movements during gastrulation. GPC3 exists as a heterodimer formed by disulfide linkage and interacts with various molecules, including DPP4, FGF2, WNT5A, WNT3A, WNT7B, hedgehog proteins SHH and IHH, and Wnt receptors FZD4, FZD7, and FZD8, showcasing its pivotal role in coordinating developmental processes.

Human

HEK293

C-mFc

P51654-1/NP_004475.1 (Q25-S550, S495A)

2719  [NCBI]

Approximately 86.11 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.