GM-CSF Protein, Human (HEK293)

The GM-CSF protein functions as a key cytokine that promotes the growth and differentiation of hematopoietic precursor cells of different lineages, such as granulocytes, macrophages, eosinophils, and erythrocytes. In its monomeric form, GM-CSF acts as a signaling molecule, orchestrating complex receptor assemblies. GM-CSF Protein, Human (HEK293) is the recombinant human-derived GM-CSF protein, expressed by HEK293 , with tag free.

GMP GM-CSF Protein functions as a pivotal cytokine, fostering the growth and differentiation of hematopoietic precursor cells spanning diverse lineages, such as granulocytes, macrophages, eosinophils, and erythrocytes. In its monomeric form, GMP GM-CSF serves as a signaling molecule, orchestrating a complex receptor assembly. This receptor complex takes the shape of a dodecamer, consisting of two head-to-head hexamers, each composed of two alpha, two beta, and two ligand subunits. This structural intricacy underscores the specificity and regulatory role of GMP GM-CSF in directing cellular responses within the hematopoietic system.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is ≤ 1.731 ng/mL, corresponding to a specific activity is ≥ 5.777×10⁵ units/mg.

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  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 22.35 pg/mL, corresponding to a specific activity is 4.474×10⁷ units/mg.

Human

HEK293

Tag Free

P04141 (A18-E144)

1437  [NCBI]

APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

Approximately 19-29 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.