GM-CSF Protein, Human (HEK293, His)

The GM-CSF protein functions as a key cytokine that promotes the growth and differentiation of hematopoietic precursor cells of different lineages, such as granulocytes, macrophages, eosinophils, and erythrocytes. In its monomeric form, GM-CSF acts as a signaling molecule, orchestrating complex receptor assemblies. GM-CSF Protein, Human (HEK293, His) is the recombinant human-derived GM-CSF protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GM-CSF Protein, Human (HEK293, His) is 127 a.a., with molecular weight of 17-30 kDa.

GMP GM-CSF Protein functions as a pivotal cytokine, fostering the growth and differentiation of hematopoietic precursor cells spanning diverse lineages, such as granulocytes, macrophages, eosinophils, and erythrocytes. In its monomeric form, GMP GM-CSF serves as a signaling molecule, orchestrating a complex receptor assembly. This receptor complex takes the shape of a dodecamer, consisting of two head-to-head hexamers, each composed of two alpha, two beta, and two ligand subunits. This structural intricacy underscores the specificity and regulatory role of GMP GM-CSF in directing cellular responses within the hematopoietic system.

The cell proliferation assay using TF-1 human erythroleukemic cells has an ED₅₀ value of 6-30 pg/mL.

Human

HEK293

C-6*His

P04141 (A18-E144)

1437  [NCBI]

APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

17-30 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.