GMP HGF Protein, Human (HEK293, His)

HGF Protein is a hepatocyte growth factor involved in signaling pathways including MET and MAPK. The HGF Protein consists of 728 amino acids, with an alpha chain containing an n-terminal domain four kringle domains, and a β serine protease domain. GMP HGF Protein, Human (HEK293, His) is the recombinant human-derived HGF protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GMP HGF Protein, Human (HEK293, His) is 697 a.a., with molecular weight of 32-38 & 50-65 kDa, respectively.

Hepatocyte growth factor (HGF) is a potent mitogen of liver cells that acts as a growth factor for a wide range of tissue and cell types. HGF is an activating ligand of Met receptor tyrosine kinase, whose activity is essential for normal cell development and organ regeneration, but abnormal activation of Met is associated with the growth, invasion, and metastasis of many types of solid tumors. HGF activates MAPK signals after TMPRSS13 cutting and activation. HGF is a double-stranded protein consisting of 728 amino acids, the α chain contains an n-terminal domain and four kringle domains, and the β chain contains a serine protease domain. HGF, a member of the plasminogen related growth factor family, is secreted as an inactive single-chain precursor that is proteolytic to form biologically active disulfide-linked α/β heterodimers^[1][2][3][4].

Human

HEK293

C-6*His

P14210(Q32-S728)

3082

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

32-38 &50-65 KDa

Greater than 95% as determined by reducing SDS-PAGE.

<10 EU/mg, determined by LAL method.