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  2. Cytokines and Growth Factors GMP-grade Proteins
  3. GMP Noggin Protein, Human (HEK293)

GMP Noggin Protein, Human (HEK293)

Cat. No.: HY-P70558G
COA Handling Instructions

Noggin protein is an important BMP inhibitor that plays an indispensable role in the neural tube, somite growth, cartilage morphogenesis, and joint formation. Its homodimeric structure promotes significant interactions with GDF5 and possibly GDF6, inhibiting chondrocyte differentiation. GMP Noggin Protein, Human (HEK293) is the recombinant human-derived Noggin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GMP Noggin Protein, Human (HEK293) is 205 a.a., with molecular weight of ~28-32 kDa.

GMP-grade recombinant proteins are produced under independent QA supervision, as well as quality records and full traceability.

For research use only. We do not sell to patients.

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50 μg $700 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

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Description

Noggin protein is an important BMP inhibitor that plays an indispensable role in the neural tube, somite growth, cartilage morphogenesis, and joint formation. Its homodimeric structure promotes significant interactions with GDF5 and possibly GDF6, inhibiting chondrocyte differentiation. GMP Noggin Protein, Human (HEK293) is the recombinant human-derived Noggin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GMP Noggin Protein, Human (HEK293) is 205 a.a., with molecular weight of ~28-32 kDa.

Background

Noggin protein emerges as a crucial inhibitor in the intricate realm of bone morphogenetic proteins (BMP) signaling, playing indispensable roles in neural tube and somite growth, as well as contributing to the intricate processes of cartilage morphogenesis and joint formation. Operating through its homodimeric structure, Noggin establishes a significant interaction with GDF5, and likely GDF6, exerting its inhibitory influence on chondrocyte differentiation. This molecular interplay underscores Noggin's pivotal position in regulating key aspects of embryonic development, emphasizing its nuanced involvement in sculpting the intricate patterns and structures critical for proper growth and morphogenesis.

Biological Activity

Measured by its ability to inhibit BMP-2-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells The ED50 for this effect is ≤0.13 μg/mL in the presence of 2000 ng/mL of Recombinant Human BMP‑2.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q13253 (Q28-C232)

Gene ID
Molecular Construction
N-term
GMP Noggin (Q28-C232)
Accession # Q13253
6*His
C-term
Synonyms
Noggin; NOG
AA Sequence

QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

Molecular Weight

Approximately 28-32 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 20mM PB, 500mM NaCl, 2mM EDTA, pH 7.4.

Endotoxin Level

<0.01 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

GMP Noggin Protein, Human (HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GMP Noggin Protein, Human (HEK293)
Cat. No.:
HY-P70558G
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