gp130/IL6ST Protein, Human (HEK293, His)

gp130/IL6ST is an important signal transduction molecule that can form high-affinity receptor complexes for cytokines such as IL6, LIF, and OSM. Upon IL6 binding, gp130/IL6ST homodimerizes and activates the JAK-MAPK and JAK-STAT3 pathways, leading to the phosphorylation of IL6ST tyrosine residues and subsequent activation of STAT3. gp130/IL6ST Protein, Human (HEK293, His) is the recombinant human-derived gp130/IL6ST protein, expressed by HEK293 , with C-His labeled tag.

gp130/IL6ST, a signal-transducing molecule, serves as a crucial component in receptor systems for various cytokines, including IL6, LIF, OSM, CNTF, IL11, CTF1, and BSF3, facilitating signal transmission. Upon binding of IL6 to IL6R, IL6ST undergoes homodimerization, forming a high-affinity receptor complex that activates the JAK-MAPK and JAK-STAT3 signaling pathways. This activation leads to the phosphorylation of IL6ST tyrosine residues, subsequently activating STAT3. Simultaneously, IL6 signaling induces the expression of cytokine receptor signaling inhibitors, SOCS1 and SOCS3, establishing a negative feedback loop to regulate JAK activity (By similarity). Furthermore, IL6ST plays a role in controlling inflammation-induced epithelial regeneration independently of STAT3 by activating the yes-associated protein 1 (YAP) and NOTCH pathways (By similarity). Acting as a receptor for the neuroprotective peptide humanin in conjunction with IL27RA/WSX1 and CNTFR, IL6ST mediates signals crucial for immune response, hematopoiesis, pain control, bone metabolism, and embryonic development (By similarity). Additionally, IL6ST is essential for the survival and differentiation of motor and sensory neurons, the expression of TRPA1 in nociceptive neurons, and the maintenance of PTH1R expression in the osteoblast lineage (By similarity). It binds to the soluble IL6:sIL6R complex, known as hyper-IL6, inhibiting IL6 trans-signaling and blocking IL11 cluster signaling through IL11R.

Human

HEK293

C-His

P40189-1 (E23-I618)

3572

78-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.