1. Recombinant Proteins
  2. Others
  3. gp15 Protein, Escherichia phage T7 (His, Strep)

gp15 Protein, Escherichia phage T7 (His, Strep)

Cat. No.: HY-P701851
Handling Instructions

Gp15 protein, part of the inner core during viral capsid formation, aids in ejecting viral DNA into the host cell. It forms a complex with gp16 and binds to both viral DNA and the host inner membrane. Gp15 interacts with gp14 and likely remains associated with gp16, controlling the translocation of DNA into the cell. gp15 Protein, Escherichia phage T7 (His, Strep) is the recombinant gp15 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of gp15 Protein, Escherichia phage T7 (His, Strep) is 746 a.a., .

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Gp15 protein, part of the inner core during viral capsid formation, aids in ejecting viral DNA into the host cell. It forms a complex with gp16 and binds to both viral DNA and the host inner membrane. Gp15 interacts with gp14 and likely remains associated with gp16, controlling the translocation of DNA into the cell. gp15 Protein, Escherichia phage T7 (His, Strep) is the recombinant gp15 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of gp15 Protein, Escherichia phage T7 (His, Strep) is 746 a.a., .

Background

Gp15, a pivotal component of the cylindrical core, plays a crucial role in the assembly of the inner surface of the capsid during capsid formation and is actively involved in the ejection of viral DNA into the host cell. This inner core comprises stacked rings of gp14, gp15, and gp16 proteins. Upon binding to the host cell surface, the internal core undergoes disassembly, leading to the ejection of gp15, along with gp14 and gp16, into the infected cell. It is likely that gp15 remains associated with gp16. The resulting gp15-gp16 complex forms interactions with both the viral DNA and the host inner membrane, potentially guiding the leading end of the genome through the periplasm and regulating the extent of DNA translocated into the host cell. Gp15 functions as a homooctamer and interacts with gp16, forming a complex after ejection, composed of a gp15 octamer and a gp16 tetramer, which likely binds both the viral DNA and the host inner membrane. Furthermore, gp15 interacts with gp14, emphasizing its integral role in the intricate orchestration of events during the viral life cycle.

Species

Others

Source

E. coli

Tag

N-Strep;N-6*His

Accession

P03725 (S2-E747)

Gene ID

1261034

Molecular Construction
N-term
Strep-6*His
gp15 (S2-E747)
Accession # P03725
C-term
Synonyms
Internal virion protein gp15; Gene product 15; Gp15
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

gp15 Protein, Escherichia phage T7 (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
gp15 Protein, Escherichia phage T7 (His, Strep)
Cat. No.:
HY-P701851
Quantity:
MCE Japan Authorized Agent: