GPT Protein, Rat (sf9, His)

GPT proteins play a key role in cellular nitrogen metabolism by catalyzing the reversible ammonia interaction between alanine and 2-oxoglutarate to produce pyruvate and glutamate products. This enzyme activity is an integral part of the dynamic balance of amino acid metabolism and contributes to the interconversion of key metabolites. GPT Protein, Rat (sf9, His) is the recombinant rat-derived GPT protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of GPT Protein, Rat (sf9, His) is 496 a.a., with molecular weight of ~48 kDa.

The GPT protein, also known as alanine transaminase or ALT, plays a crucial role in cellular nitrogen metabolism by catalyzing the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This enzymatic activity is central to the interconversion of amino acids and is particularly important for the regulation of nitrogen balance within the cell. GPT is actively involved in liver gluconeogenesis, contributing to the synthesis of glucose from precursors transported from skeletal muscles. The reversible transamination reactions mediated by GPT are integral to various metabolic pathways, emphasizing its significance in amino acid metabolism and glucose homeostasis. Understanding the functions of GPT provides insights into the dynamic regulation of nitrogen and carbon flux in cellular metabolism.

Rat

Sf9 insect cells

C-His

P25409 (M1-S496)

81670  [NCBI]

Approximately 48 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.