GPT1 Protein, Human (His)

The GPT1 protein is a key player that catalyzes the reversible cleavage of L-arginine succinate, which is critical in the urea cycle, aids in hepatic nitrogen detoxification and promotes de novo L-arginine synthesis. GPT1 regulates intracellular and extracellular L-arginine pools, which are critical for nitrogen homeostasis. GPT1 Protein, Human (His) is the recombinant human-derived GPT1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GPT1 Protein, Human (His) is 495 a.a., with molecular weight of 58.5 kDa.

Argininosuccinate lyase (ASL) protein serves a pivotal role by catalyzing the reversible cleavage of L-argininosuccinate into fumarate and L-arginine, a crucial step in the urea cycle. This process primarily contributes to hepatic nitrogen detoxification, leading to the excretion of urea, and facilitates de novo L-arginine synthesis in nonhepatic tissues. ASL emerges as an essential regulator of both intracellular and extracellular L-arginine pools, playing a critical role in maintaining nitrogen homeostasis. As part of the citrulline-nitric oxide cycle, ASL forms tissue-specific multiprotein complexes with argininosuccinate synthase (ASS1), transport protein SLC7A1, and nitric oxide synthase (NOS1, NOS2, or NOS3). This complex allows for cell-autonomous L-arginine synthesis while participating in the channeling of extracellular L-arginine to the nitric oxide synthesis pathway, showcasing the multifaceted regulatory functions of ASL in nitrogen metabolism and cellular signaling.

Human

E. coli

N-6*His

P24298 (A2-S496)

2875

58.5kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.