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  3. Enzymes & Regulators
  4. GZF1 Protein, Human (Sf9, His, Strep)

GZF1 Protein, Human (Sf9, His, Strep)

Cat. No.: HY-P701525
Handling Instructions

GZF1, a transcriptional repressor, regulates gene expression by binding to the GZF1 responsive element (GRE) with the consensus sequence 5'-TGCGCN[TG][CA]TATA-3'. Its role includes potential regulation of VSX2/HOX10 expression, and it interacts with NCL, suggesting a molecular association that contributes to its transcriptional modulatory activities. GZF1 Protein, Human (Sf9, His, Strep) is the recombinant human-derived GZF1 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of GZF1 Protein, Human (Sf9, His, Strep) is 710 a.a., .

For research use only. We do not sell to patients.

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Description

GZF1, a transcriptional repressor, regulates gene expression by binding to the GZF1 responsive element (GRE) with the consensus sequence 5'-TGCGCN[TG][CA]TATA-3'. Its role includes potential regulation of VSX2/HOX10 expression, and it interacts with NCL, suggesting a molecular association that contributes to its transcriptional modulatory activities. GZF1 Protein, Human (Sf9, His, Strep) is the recombinant human-derived GZF1 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of GZF1 Protein, Human (Sf9, His, Strep) is 710 a.a., .
Background

GZF1, a transcriptional repressor, exerts its regulatory function by binding to the GZF1 responsive element (GRE) with the consensus sequence 5'-TGCGCN[TG][CA]TATA-3'. This protein is implicated in the potential regulation of VSX2/HOX10 expression. Its interaction with NCL highlights a molecular association that contributes to its transcriptional modulatory activities.
Species

Human
Source

Sf9 insect cells
Tag

N-Strep;N-8*His
Accession

Q9H116 (E2-E711)
Gene ID

64412
Molecular Construction
N-term
8*His-Strep
GZF1 (E2-E711)
Accession # Q9H116
C-term
Synonyms
GZF1; GDNF-inducible zinc finger protein 1; Zinc finger and BTB domain-containing protein 23; Zinc finger protein 336
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

GZF1 Protein, Human (Sf9, His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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