HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His)

Hemagglutinin protein (HA) is a class I viral fusion protein that binds to sialic acid-containing receptors and initiates viral attachment to cells. This binding promotes internalization of virions through various endocytic pathways. HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

The Hemagglutinin Protein (HA), a class I viral fusion protein, binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle to the cell. This attachment leads to virion internalization through clathrin-dependent endocytosis or clathrin- and caveolin-independent pathways. HA also plays a crucial role in determining the host range restriction and virulence of the virus. By mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane, HA enables the penetration of the virus into the cell cytoplasm. In the low pH environment of endosomes, HA2 undergoes an irreversible conformational change, releasing the fusion hydrophobic peptide and forming a competent fusion pore. HA exists as a homotrimer composed of disulfide-linked HA1-HA2 subunits and interacts with human CACNA1C.

Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.02-0.4 μg/mL for 1% GRBC.

Virus

Sf9 insect cells

C-His

P03452 (D18-Q528)

956529  [NCBI]

Approximately 59.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.