HACE1 Protein, Human (His)

The HACE1 protein is an E3 ubiquitin protein ligase that regulates Golgi membrane fusion and small GTPases. HACE1 Protein, Human (His) is the recombinant human-derived HACE1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HACE1 Protein, Human (His) is 908 a.a., .

HACE1, an E3 ubiquitin-protein ligase, intricately participates in Golgi membrane fusion and the regulation of small GTPases. Its crucial role in Golgi membrane dynamics during the cell cycle involves recruitment to the Golgi membrane by Rab proteins, where it orchestrates postmitotic Golgi membrane fusion. During mitotic Golgi disassembly, HACE1 mediates ubiquitination, serving as a pivotal signal for Golgi reassembly in the subsequent stages of cell division. Notably, HACE1 exhibits specificity in its interaction with GTP-bound RAC1, leading to the ubiquitination and subsequent degradation of active RAC1. This function positions HACE1 as a significant contributor to host defense against pathogens. Additionally, HACE1 may extend its influence as a transcription regulator through interaction with RARB.

Human

E. coli

N-6*His

Q8IYU2 (E2-A909)

57531

Approximately 104.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

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Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.