HAMP protein, Larimichthys crocea

HAMP protein is a key signaling molecule for iron homeostasis and cooperates with HFE to regulate intestinal iron absorption and macrophage iron storage. HAMP has significant antibacterial effects and shows strong antibacterial activity against Gram-negative bacteria such as Vibrio alginolyticus and Gram-positive bacteria such as Staphylococcus aureus. HAMP protein, Larimichthys crocea is the recombinant HAMP protein, expressed by E. coli , with tag free. The total length of HAMP protein, Larimichthys crocea is 21 a.a., with molecular weight of ~2.5 kDa.

HAMP protein appears to function as a signaling molecule crucial for maintaining iron homeostasis, working in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Its antimicrobial properties are notable, demonstrating robust antibacterial activity against various Gram-negative bacteria, including V.alginolyticus, V.fluvialis, V.harveyis, and V.parahaemolyticus, with varying minimum inhibitory concentrations (MICs). Additionally, HAMP displays antibacterial activity against Gram-positive bacteria such as B.cereus, B.subtilis, C.glutamicum, M.luteus, M.lysodeikticus, S.aureus, and S.epidermis. Furthermore, it exhibits antifungal activity against A.niger, F.graminearum, and F.solani, while lacking antifungal effects against the yeasts P.pastoris GS115 and C.albicans. These diverse activities underscore the multifaceted role of HAMP in host defense mechanisms against microbial challenges.

Others

E. coli

Tag Free

A1Z0M0 (R65-F85)

104926498  [NCBI]

RCRFCCRCCPRMRGCGICCRF

Approximately 2.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.