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  4. HAMP protein, Larimichthys crocea

HAMP protein, Larimichthys crocea

Cat. No.: HY-P72221
COA Handling Instructions

HAMP protein is a key signaling molecule for iron homeostasis and cooperates with HFE to regulate intestinal iron absorption and macrophage iron storage. HAMP has significant antibacterial effects and shows strong antibacterial activity against Gram-negative bacteria such as Vibrio alginolyticus and Gram-positive bacteria such as Staphylococcus aureus. HAMP protein, Larimichthys crocea is the recombinant HAMP protein, expressed by E. coli , with tag free. The total length of HAMP protein, Larimichthys crocea is 21 a.a., with molecular weight of ~2.5 kDa.

For research use only. We do not sell to patients.

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Description

HAMP protein is a key signaling molecule for iron homeostasis and cooperates with HFE to regulate intestinal iron absorption and macrophage iron storage. HAMP has significant antibacterial effects and shows strong antibacterial activity against Gram-negative bacteria such as Vibrio alginolyticus and Gram-positive bacteria such as Staphylococcus aureus. HAMP protein, Larimichthys crocea is the recombinant HAMP protein, expressed by E. coli , with tag free. The total length of HAMP protein, Larimichthys crocea is 21 a.a., with molecular weight of ~2.5 kDa.

Background

HAMP protein appears to function as a signaling molecule crucial for maintaining iron homeostasis, working in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Its antimicrobial properties are notable, demonstrating robust antibacterial activity against various Gram-negative bacteria, including V.alginolyticus, V.fluvialis, V.harveyis, and V.parahaemolyticus, with varying minimum inhibitory concentrations (MICs). Additionally, HAMP displays antibacterial activity against Gram-positive bacteria such as B.cereus, B.subtilis, C.glutamicum, M.luteus, M.lysodeikticus, S.aureus, and S.epidermis. Furthermore, it exhibits antifungal activity against A.niger, F.graminearum, and F.solani, while lacking antifungal effects against the yeasts P.pastoris GS115 and C.albicans. These diverse activities underscore the multifaceted role of HAMP in host defense mechanisms against microbial challenges.

Species

Others

Source

E. coli

Tag

Tag Free

Accession

A1Z0M0 (R65-F85)

Gene ID

104926498  [NCBI]

Molecular Construction
N-term
HAMP (R65-F85)
Accession # A1Z0M0
C-term
Synonyms
hamp; Hepcidin
AA Sequence

RCRFCCRCCPRMRGCGICCRF

Molecular Weight

Approximately 2.5 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

HAMP protein, Larimichthys crocea Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HAMP protein, Larimichthys crocea
Cat. No.:
HY-P72221
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