HCMV Glycoprotein B/gB Protein (HEK293, Fc)

The gB Protein is an envelope glycoprotein that plays a role in host cell entry, cell-to-cell viral transmission, and infected cell fusion.gB Protein interacts with heparin sulfate proteoglycan and forms an association with gH/gL heterodimer.HCMV Glycoprotein B/gB Protein (HEK293, Fc) is a recombinant Virus-derived HCMV Glycoprotein B/gB protein(with cleavage site mutated RTKR to TTQT), expressed by HEK293 , with C-hFc labeled tag.HCMV Glycoprotein B/gB Protein (HEK293, Fc), has molecular weight of 160-170 kDa.

gB is an envelope glycoprotein that plays a role in host cell entry, cell-to-cell viral transmission, and infected cell fusion. gB is involved in initial attachment through a gM/gN complex bound to heparin sulfate, which binds heparin with higher affinity. gB plays a key role in the fusion of viruses and cell membranes, facilitating the entry of viruses into host cells. The complex fusion mechanisms required for this process include at least gB and gH/gL heterodimers. gB interacts with host integrins ITGB1, PDGFRA, and EGFR, possibly entering the receptor as post-attachment^[1][2][3].

Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA.

Virus

HEK293

C-hFc

AAA45920 (S25-K700&R777-V907, with cleavage site mutated RTKR to TTQT)

/

160-170 kDa

Greater than 80% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.