HCoV-229E Spike/S Protein (APT69883, sf9, His)

Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses. The CoVs S-protein mediates receptor binding and fusion of the viral and host cell membranes. HCoV-229E is an alpha-coronaviruse that uses different host proteins as receptors.In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation. HCoV-229E exploits trypsin, cathepsin L and TMPRSS2 to complete the fusion activation mediated by the S protein^[1][2]. HCoV-229E Spike/S Protein (APT69883, sf9, His) is the recombinant Virus-derived HCoV-229E Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag.

Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses (~26–32 kb) belonging to the order Nidovirales1. Coronaviruses spike (S) glycoproteins mediate viral entry into host cells by binding to host receptors. To date, there are seven coronaviruses, namely the alphacoronaviruses HCoV-229E and HCoV-NL63, the betacoronaviruses HCoV-OC43, HCoV-HKU1, SARS-CoV, MERS-CoV and the emerging coronavirus (SARS-CoV-2). Moreover, fusion activation of HCoV-229E may be highly reliant on cleavage of the S2´ trigger loop (GSR685↓V686AG)^[1][2].

Virus

Sf9 insect cells

C-His

APT69883 (C16-W1115)

/

Approximately 122.1 kDa.

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.