HCoV-HKU1 Spike/S1 Protein (Q0ZME7, HEK293, His)

HCoV-HKU1 Spike Proteinas initiates viral infection by binding to host receptors, facilitating virion attachment to the cell membrane. Operating as a class I viral fusion protein, it orchestrates fusion between the virion and cellular membranes through a series of conformational states. These include a pre-fusion native state, a pre-hairpin intermediate state, and a post-fusion hairpin state. The structural rearrangement during fusion, involving heptad repeats, is essential for merging viral and target cell membranes. HCoV-HKU1 Spike/S1 Protein (Q0ZME7, HEK293, His) is the recombinant Virus-derived HCoV-HKU1 Spike/S1 protein, expressed by HEK293, with C-His labeled tag. The total length of HCoV-HKU1 Spike/S1 Protein (Q0ZME7, HEK293, His) is 744 a.a., with molecular weight of ~85.2 KDa.

The HCoV-HKU1 Spike/S1 Protein plays a pivotal role in the viral infection process by attaching the virion to the cell membrane through interaction with host receptors, thereby initiating infection. Functioning as a class I viral fusion protein, it mediates the fusion of the virion with cellular membranes. The protein undergoes at least three conformational states: a pre-fusion native state, a pre-hairpin intermediate state, and a post-fusion hairpin state. During the fusion process between viral and target cell membranes, the coiled coil regions (heptad repeats) adopt a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. This structural rearrangement is crucial for driving the apposition and subsequent fusion of viral and target cell membranes.

Virus

HEK293

C-His

Q0ZME7 (A13-R756)

/

Approximately 85.2 kDa.

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.