HCoV-NL63 Spike/S Protein (APF29071, sf9, His)

HCoV-NL63 is a coronavirus, specifically a filovirus from the genus coronavirus A. HCoV-NL63 is a coated, positive single-stranded RNA virus that enters host cells by binding to ACE2. HCoV-NL63 is mainly found in young children, the elderly, and immunocompromised patients with acute respiratory diseases. HCoV-NL63 Spike/S Protein (APF29071, sf9, His) is the recombinant Virus-derived HCoV-NL63 Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HCoV-NL63 Spike/S Protein (APF29071, sf9, His) is 1296 a.a., with molecular weight of ~142.4 KDa.

HCoV-NL63 is a coronavirus, specifically a filovirus from the genus coronavirus A. The virus is a coated, positive single-stranded RNA virus that enters host cells by binding to ACE2. The S1 region attaches virions to the cell membrane through interactions with host receptors, triggering infection. Binding to the receptor may cause conformational changes of S glycoprotein, reveal fusion peptide in the S2 region, and activate membrane fusion. The S2 region belongs to the Class I virus fusion protein. The virus is mainly found in young children, the elderly and immunocompromised patients with acute respiratory illness^[1][2][3].

Virus

Sf9 insect cells

C-His

APF29071 (M1-P1296)

/

Approximately 142.4 kDa.

Greater than 80% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.