1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. HDAC8 Protein, Human (sf9)

HDAC8 Protein, Human (sf9)

Cat. No.: HY-P702858
COA Handling Instructions

The HDAC8 protein is a histone deacetylase that has been shown to be critical for lysine deacetylation on core histones (H2A, H2B, H3, H4). This enzymatic activity initiates epigenetic repression that affects transcription, cell cycle, and developmental events. HDAC8 Protein, Human (sf9) is the recombinant human-derived HDAC8 protein, expressed by Sf9 insect cells , with no tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
10 μg $185 In-stock
50 μg $480 In-stock
100 μg $770 In-stock
> 100 μg   Get quote  

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  • Biological Activity

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Description

The HDAC8 protein is a histone deacetylase that has been shown to be critical for lysine deacetylation on core histones (H2A, H2B, H3, H4). This enzymatic activity initiates epigenetic repression that affects transcription, cell cycle, and developmental events. HDAC8 Protein, Human (sf9) is the recombinant human-derived HDAC8 protein, expressed by Sf9 insect cells , with no tag.

Background

HDAC8 Protein, a histone deacetylase, catalyzes the deacetylation of lysine residues located on the N-terminal region of core histones, namely H2A, H2B, H3, and H4, as supported by various studies. This enzymatic activity is crucial in initiating epigenetic repression, playing pivotal roles in transcriptional regulation, cell cycle progression, and developmental events. Acting within large multiprotein complexes, histone deacetylases like HDAC8 contribute to the intricate regulation of chromatin structure and function. Moreover, HDAC8 is implicated in the deacetylation of the cohesin complex protein SMC3, influencing the release of cohesin complexes from chromatin. Notably, HDAC8 may also play a role in smooth muscle cell contractility. Expanding its enzymatic repertoire, HDAC8 exhibits protein-lysine deacylase activity, specifically functioning as a protein decrotonylase by mediating the decrotonylation of histones, particularly crotonylation at lysine residues ((2E)-butenoyl).

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

Tag Free

Accession

Q9BY41-1 (M1-V377)

Gene ID

55869

Molecular Construction
N-term
HDAC8 (M1-V377)
Accession # Q9BY41-1
C-term
Synonyms
Histone deacetylase 8; HD8; HDAC8; HDACL1; CDA07
AA Sequence

MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV

Molecular Weight

Approximately 42.5 KDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

HDAC8 Protein, Human (sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HDAC8 Protein, Human (sf9)
Cat. No.:
HY-P702858
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