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HE4/WFDC2 Protein, Canine (HEK293, Fc)

Cat. No.: HY-P75803
COA Handling Instructions

HE4/WFDC2 protein acts as a broad-spectrum protease inhibitor and may affect a variety of cellular processes. Its effect on sperm maturation suggests its role in reproductive mechanisms. HE4/WFDC2 Protein, Canine (HEK293, Fc) is the recombinant canine-derived HE4/WFDC2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of HE4/WFDC2 Protein, Canine (HEK293, Fc) is 97 a.a., with molecular weight of ~43-50 kDa.

For research use only. We do not sell to patients.

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Description

HE4/WFDC2 protein acts as a broad-spectrum protease inhibitor and may affect a variety of cellular processes. Its effect on sperm maturation suggests its role in reproductive mechanisms. HE4/WFDC2 Protein, Canine (HEK293, Fc) is the recombinant canine-derived HE4/WFDC2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of HE4/WFDC2 Protein, Canine (HEK293, Fc) is 97 a.a., with molecular weight of ~43-50 kDa.

Background

HE4/WFDC2 Protein serves as a broad-spectrum protease inhibitor, potentially contributing to diverse cellular processes. Its putative role in sperm maturation suggests involvement in reproductive mechanisms. Structurally, it forms a homotrimer through disulfide linkages, highlighting its oligomeric nature. The multifaceted function of HE4/WFDC2 in protease regulation and potential impact on reproductive processes underscores its significance in cellular homeostasis.

Species

Canine

Source

HEK293

Tag

C-hFc

Accession

Q28894/NP_001003241.1 (G28-F124)

Gene ID
Molecular Construction
N-term
HE4 (G28-F124)
Accession # Q28894/NP_001003241.1
hFc
C-term
Synonyms
WAP four-disulfide core domain protein 2; CE4; WFDC2
AA Sequence

GEVEKTGVCPQLQADLNCTQECVSDAQCADNLKCCQAGCATICHLPNEKEGSCPQVNTDFPQLGLCQDQCQVDSHCPGLLKCCYNGCGKVSCVTPIF

Molecular Weight

Approximately 43-50 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

HE4/WFDC2 Protein, Canine (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HE4/WFDC2 Protein, Canine (HEK293, Fc)
Cat. No.:
HY-P75803
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