HA/Hemagglutinin Protein, H10N3 (ACT84107, sf9, His)

Influenza A virus is a single-stranded, negative-sense, segmented RNA virus of the family Orthomyxoviridae. Its genome harbours eight viral RNA (vRNA) segments encoding at least 17 proteins. HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins. H10N3 avian influenza viruses (AIVs) bind to α-2, 3-linked sialic acids with high affinity and to α-2, 6-linked sialic acids with affinity^[1][2][3]. HA/Hemagglutinin Protein, H10N3 (ACT84107, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

Influenza A viruses are categorized into 18 different HA subtypes and 11 different NA subtypes based on the different antigenicity of their two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Influenza viruses are prone to genetic mutations during replication, and sometimes one or two amino acid mutations can cause huge biological changes^[1].
HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins. Particularly, influenza viruses predominantly bind to α2,3-linked sialic acid (SA) receptors abundantly located in the lower respiratory tract of birds and the intestinal tract with respect to avian influenza viruses, to α2,6-linked SA receptors. Upon virus attachment, a conformational change in the HA protein is required for the fusion reaction between the viral and endosomal membranes, after which viral ribonucleoproteins are released in the cytoplasm of the infected cell^[2].

Virus

Sf9 insect cells

C-His

ACT84107 (L17-V525)

/

Approximately 64 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.