HA/Hemagglutinin Protein, H5N1 (AAF02306, HEK293, His)

The hemagglutinin (HA) protein attaches viral particles to host cells by binding to sialic acid-containing receptors and induces viral particle internalization through clathrin-dependent endocytosis.HA also promotes an alternative clathrin- and caveolin-independent pathway for some virions.HA/Hemagglutinin Protein, H5N1 (AAF02306, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

The Hemagglutinin (HA) protein plays a crucial role in the attachment of virus particles to host cells by binding to sialic acid-containing receptors on the cell surface. This interaction not only induces virion internalization through clathrin-dependent endocytosis but also facilitates an alternative clathrin- and caveolin-independent pathway for about one-third of the virus particles. HA is a Class I viral fusion protein responsible for penetrating the cell cytoplasm by mediating the fusion of the virus particle's membrane with the endosomal membrane. The low pH environment in endosomes triggers an irreversible conformational change in HA2, leading to the release of the fusion hydrophobic peptide. The cooperative action of several trimers is necessary to form a competent fusion pore, highlighting the intricate role of HA in host range restriction and virulence determination.

Virus

HEK293

C-His

AAF02306 (R23-Q539, with cleavage site mutated RRRKKR to TETR)

/

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.