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Hemopexin Protein, Mouse (HEK293, His)

Cat. No.: HY-P70884A
COA Handling Instructions

Hemopexin Protein plays a crucial role in binding heme, transporting it to the liver for breakdown, and facilitating iron recovery. After this cycle, hemopexin re-enters circulation, emphasizing its pivotal role in regulating heme and iron homeostasis. Hemopexin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hemopexin protein, expressed by HEK293, with C-6*His labeled tag. The total length of Hemopexin Protein, Mouse (HEK293, His) is 437 a.a., with molecular weight of approximately 60-84 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Hemopexin Protein plays a crucial role in binding heme, transporting it to the liver for breakdown, and facilitating iron recovery. After this cycle, hemopexin re-enters circulation, emphasizing its pivotal role in regulating heme and iron homeostasis. Hemopexin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hemopexin protein, expressed by HEK293, with C-6*His labeled tag. The total length of Hemopexin Protein, Mouse (HEK293, His) is 437 a.a., with molecular weight of approximately 60-84 kDa.

Background

The Hemopexin Protein serves a vital function by binding to heme, facilitating its transport to the liver for breakdown, and enabling the recovery of iron. Following this process, the now free hemopexin re-enters the circulation, highlighting its role as a crucial mediator in the regulation of heme and iron homeostasis.

Biological Activity

Measured by its ability to bind protoporphyrin IX (PPP-IX). Recombinant Mouse Hemopexin binds 10.77 μM PPP-IX, resulting in a 50% decrease in the fluorescence signal of rhHemopexin as measured under the described conditions.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

Q91X72(S24-Q460)

Gene ID

15458

Molecular Construction
N-term
Hemopexin (S24-Q460)
Accession # Q91X72
6*His
C-term
AA Sequence

SPLPTANGRVAEVENGTKPDSDVPEHCLDTWSFDAATMDHNGTMLFFKGEFVWRGHSGTRELISARWKNPITSVDAAFRGPDSVFLIKEDKVWVYPPEKKENGYPKLFQEEFPGIPYPPDAAVECHRGECQSEGVLFFQGNRKWFWDFATRTQKERSWSTVGNCTAALRWLERYYCFQGNKFLRFNPVTGEVPPRYPLDARDYFVSCPGRGHGRPRNGTAHGNSTHPMHSRCSPDPGLTALLSDHRGATYAFTGSHYWRLDSSRDGWHSWPIAHHWPQGPSTVDAAFSWDDKVYLIQGTQVYVFLTKGGNNLVSGYPKRLEKELGSPPGISLETIDAAFSCPGSSRLYVSSGRRLWWLDLKSGAQATWTEVSWPHEKVDGALCLDKSLGPNTCSSNGSSLYFIHGPNLYCYSSIDKLNAAKSLPQPQKVNSILGCSQ

Molecular Weight

approximately 60-84 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Hemopexin Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Hemopexin Protein, Mouse (HEK293, His)
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HY-P70884A
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