Hemopexin Protein, Mouse (HEK293, His)

Hemopexin Protein plays a crucial role in binding heme, transporting it to the liver for breakdown, and facilitating iron recovery. After this cycle, hemopexin re-enters circulation, emphasizing its pivotal role in regulating heme and iron homeostasis. Hemopexin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hemopexin protein, expressed by HEK293, with C-6*His labeled tag. The total length of Hemopexin Protein, Mouse (HEK293, His) is 437 a.a., with molecular weight of approximately 60-84 kDa.

The Hemopexin Protein serves a vital function by binding to heme, facilitating its transport to the liver for breakdown, and enabling the recovery of iron. Following this process, the now free hemopexin re-enters the circulation, highlighting its role as a crucial mediator in the regulation of heme and iron homeostasis.

Measured by its ability to bind protoporphyrin IX (PPP-IX). Recombinant Mouse Hemopexin binds 10.77 μM PPP-IX, resulting in a 50% decrease in the fluorescence signal of rhHemopexin as measured under the described conditions.

Mouse

HEK293

C-6*His

Q91X72(S24-Q460)

15458

SPLPTANGRVAEVENGTKPDSDVPEHCLDTWSFDAATMDHNGTMLFFKGEFVWRGHSGTRELISARWKNPITSVDAAFRGPDSVFLIKEDKVWVYPPEKKENGYPKLFQEEFPGIPYPPDAAVECHRGECQSEGVLFFQGNRKWFWDFATRTQKERSWSTVGNCTAALRWLERYYCFQGNKFLRFNPVTGEVPPRYPLDARDYFVSCPGRGHGRPRNGTAHGNSTHPMHSRCSPDPGLTALLSDHRGATYAFTGSHYWRLDSSRDGWHSWPIAHHWPQGPSTVDAAFSWDDKVYLIQGTQVYVFLTKGGNNLVSGYPKRLEKELGSPPGISLETIDAAFSCPGSSRLYVSSGRRLWWLDLKSGAQATWTEVSWPHEKVDGALCLDKSLGPNTCSSNGSSLYFIHGPNLYCYSSIDKLNAAKSLPQPQKVNSILGCSQ

approximately 60-84 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.