Heparinase I Protein, B. thetaiotaomicron (His)

Heparinase I protein is an enzyme that degrades heparin and heparan sulfate and can modify extracellular matrix components. In addition to degradation, it also releases growth factors that bind to heparin, affecting the extracellular environment. Heparinase I Protein, B. thetaiotaomicron (His) is the recombinant Heparinase I protein, expressed by E. coli , with N-His labeled tag.

Heparinase I Protein functions as an enzyme with the capacity to degrade heparin and heparan sulfate, showcasing its role in modifying the extracellular matrix components. Beyond its degradative activity, Heparinase I is also implicated in the release of growth factors that are bound to heparin from the extracellular matrix. This multifaceted enzyme plays a crucial role in the regulation of the extracellular environment, contributing to the dynamic interactions between cells and their surrounding matrix. The ability of Heparinase I to target heparin and heparan sulfate underscores its significance in various biological processes, particularly in the modulation of growth factor availability and signaling within the cellular microenvironment.

Measured by its ability to liberate oligosaccharides from heparin that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 15778.03 pmol/min/µg.

Others

E. coli

N-6*His

WP_011109372.1(M1-R376)

1073402

MLTAQTKNTQTLMPLTERVNVQADSARINQIIDGCWVAVGTNKPHAIQRDFTNLFDGKPSYRFELKTEDNTLEGYAKGETKGRAEFSYCYATSDDFRGLPADVYQKAQITKTVYHHGKGACPQGSSRDYEFSVYIPSSLDSNVSTIFAQWHGMPDRTLVQTPQGEVKKLTVDEFVELEKTTFFKKNVGHEKVARLDKQGNPVKDKNGKPVYKAGKPNGWLVEQGGYPPLAFGFSGGLFYIKANSDRKWLTDKDDRCNANPGKTPVMKPLTSEYKASTIAYKLPFADFPKDCWITFRVHIDWTVYGKEAETIVKPGMLDVRMDYQEQGKKVSKHIVDNEKILIGRNDEDGYYFKFGIYRVGDSTVPVCYNLAGYSER

Approximately 45 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.