Heparinase II Protein, P. heparinus (His)

Heparinase II protein is an enzyme that cleaves heparin and heparan sulfate glycosaminoglycans using a beta elimination mechanism. Specifically, it cleaves heparin at the α-D-GlcNp2S6S(1->4) α-L-IdoAp2S site and at the α-D-GlcNp2Ac(or 2S)6OH(1->4)beta-D-GlcAp position Site of cleavage of heparan sulfate. Heparinase II Protein, P. heparinus (His) is the recombinant Heparinase II protein, expressed by E. coli , with N-His labeled tag. The total length of Heparinase II Protein, P. heparinus (His) is 747 a.a., with molecular weight of 66-75 kDa.

Heparinase II is a protein with the remarkable ability to cleave both heparin and heparan sulfate glycosaminoglycans using a beta-elimination mechanism. This enzymatic process involves the cleavage of heparin at alpha-D-GlcNp2S6S(1->4) alpha-L-IdoAp2S and heparan sulfate at alpha-D-GlcNp2Ac(or 2S)6OH(1->4)beta-D-GlcAp. By catalyzing these specific cleavage reactions, Heparinase II plays a crucial role in the structural modification and degradation of these complex glycosaminoglycans. The enzyme's ability to target distinct glycosidic linkages in heparin and heparan sulfate makes it a valuable tool in the study of these polysaccharides, contributing to our understanding of their biological functions and potential therapeutic applications (

Measured by its ability to liberate oligosaccharides from 3 mg/mL heparin. The specific activity is 808.9 pmol/min/µg that incubate at room temperature in kinetic mode for 5 minutes.

Others

E. coli

N-6*His

C6XZB6/WP_015808224.1 (Q26-R772)

/

QTKADVVWKDVDGVSMPIPPKTHPRLYLREQQVPDLKNRMNDPKLKKVWADMIKMQEDWKPADIPEVKDFRFYFNQKGLTVRVELMALNYLMTKDPKVGREAITSIIDTLETATFKPAGDISRGIGLFMVTGAIVYDWCYDQLKPEEKTRFVKAFVRLAKMLECGYPPVKDKSIVGHASEWMIMRDLLSVGIAIYDEFPEMYNLAAGRFFKEHLVARNWFYPSHNYHQGMSYLNVRFTNDLFALWILDRMGAGNVFNPGQQFILYDAIYKRRPDGQILAGGDVDYSRKKPKYYTMPALLAGSYYKDEYLNYEFLKDPNVEPHCKLFEFLWRDTQLGSRKPDDLPLSRYSGSPFGWMIARTGWGPESVIAEMKVNEYSFLNHQHQDAGAFQIYYKGPLAIDAGSYTGSSGGYNSPHNKNFFKRTIAHNSLLIYDPKETFSSSGYGGSDHTDFAANDGGQRLPGKGWIAPRDLKEMLAGDFRTGKILAQGFGPDNQTPDYTYLKGDITAAYSAKVKEVKRSFLFLNLKDAKVPAAMIVFDKVVASNPDFKKFWLLHSIEQPEIKGNQITIKRTKNGDSGMLVNTALLPDAANSNITSIGGKGKDFWVFGTNYTNDPKPGTDEALERGEWRVEITPKKAAAEDYYLNVIQIADNTQQKLHEVKRIDGDKVVGVQLADRIVTFSKTSETVDRPFGFSVVGKGTFKFVMTDLLPGTWQVLKDGKILYPALSAKGDDGALYFEGTEGTYRFLR

85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of PBS.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.