Envelope Glycoprotein E1 Protein, HCV (HEK293, His)

Envelope Glycoprotein E1 Protein, one of two subunits of the envelope glycoprotein found in the hepatitis C virus, is a type 1 transmembrane protein.It associates with envelope glycoprotein E2 as a noncovalent heterodimer, which mediates virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane.E1/E2 heterodimer binds host apolipoproteins.And it also binds to CD81 and activates the epithelial growth factor receptor (EGFR) signaling pathway.Envelope Glycoprotein E1 Protein, HCV (HEK293, His) is the recombinant Virus-derived Envelope Glycoprotein E1 protein, expressed by HEK293 , with N-His labeled tag.

Envelope Glycoprotein E1, one of two subunits of the envelope glycoprotein found in the hepatitis C virus, is a type 1 transmembrane protein with a highly glycosylated N-terminal ectodomain and a C-terminal hydrophobic anchor. Envelope Glycoprotein E1 associates with envelope glycoprotein E2 as a noncovalent heterodimer, which mediates virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. Envelope Glycoprotein E1 helps the virus attach to the membrane of the targeted cell. In other envelope virus Envelope Glycoprotein E1 has a similar role in helping the virus get into the cell. E1/E2 heterodimer is essential for HCV entry. E1/E2 heterodimer binds host apolipoproteins such as APOB and APOE thereby forming a lipo-viro-particle (LVP). Furthermore, association of APOE with LVP allows the initial virus attachment to cell surface receptors such as the heparan sulfate proteoglycans (HSPGs), syndecan-1 (SDC1), syndecan-1 (SDC2), the low-density lipoprotein receptor (LDLR) and scavenger receptor class B type I (SCARB1). And E1/E2 heterodimer binds to CD81 and activates the epithelial growth factor receptor (EGFR) signaling pathway^[1][2][3][4].

Virus

HEK293

N-His

AAC15725 (Y192-I340)

/

Approximately 18.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.