HGF Protein, Canine (HEK293)

HGF protein is a potent hepatocyte mitogen that stimulates mature hepatocyte proliferation and exhibits multiple growth factor properties in different tissues. As a ligand of MET receptor tyrosine kinase, HGF initiates downstream signaling cascades upon binding, activating MAPK signaling after TMPRSS13 cleavage. HGF Protein, Canine (HEK293) is the recombinant canine-derived HGF protein, expressed by HEK293 , with tag free.

HGF Protein emerges as a potent mitogen with hepatotrophic properties, specifically promoting the proliferation of mature parenchymal hepatocyte cells. Beyond its hepatocyte-centric functions, HGF acts as a versatile growth factor, exerting its effects across a broad spectrum of tissues and cell types. As an activating ligand for the receptor tyrosine kinase MET, HGF binds to MET, facilitating receptor dimerization and initiating downstream signaling cascades. Furthermore, following TMPRSS13 cleavage and activation, HGF activates MAPK signaling, contributing to its diverse cellular effects. Structurally, HGF exists as a dimer composed of an alpha chain and a beta chain linked by a disulfide bond. Notably, it interacts with SRPX2, enhancing HGF's mitogenic activity. This multifaceted functionality underscores the pivotal role of HGF in cellular growth, tissue homeostasis, and signal transduction pathways.

1.Measured by its ability to compete with biotinylated Recombinant Canine HGF for binding to immobilized Recombinant Canine HGF R in a functional ELISA .The ED₅₀ for this effect is 157.3ng/mL.
2.Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED₅₀ for this effect is 14.61 ng/mL, corresponding to a specific activity is 6.845×10⁴ units/mg.

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  Measured by its ability to compete with biotinylated Recombinant Canine HGF for binding to immobilized Recombinant Canine HGF R in a functional ELISA .The ED₅₀ for this effect is 157.3 ng/mL.

Canine

HEK293

Tag Free

Q867B7/NP_001002964.1 (Q32-S730)

403441  [NCBI]

QKKRRNTLHEFKKSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMTSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDVPMETTECIQGQGEGYRGTINTIWNGVPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWEKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPIFRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRNRDLKDYEAWLGIHDVHGKGDEKRKQVLNVSQLVYGPEGSDLVLLKLARPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGSINFDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKIQQS

Approximately 93-97 kDa, 60-62 kDa and 32-35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 6.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.