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HIST3H2A Protein, Human

Cat. No.: HY-P76382
COA Handling Instructions

The HIST3H2A protein is a core component of nucleosomes that compact DNA into chromatin. HIST3H2A Protein, Human is the recombinant human-derived HIST3H2A protein, expressed by E. coli , with tag free. The total length of HIST3H2A Protein, Human is 129 a.a., with molecular weight of 15-20 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The HIST3H2A protein is a core component of nucleosomes that compact DNA into chromatin. HIST3H2A Protein, Human is the recombinant human-derived HIST3H2A protein, expressed by E. coli , with tag free. The total length of HIST3H2A Protein, Human is 129 a.a., with molecular weight of 15-20 KDa.

Background

HIST3H2A, as a core component of the nucleosome, participates in the essential process of wrapping and compacting DNA into chromatin, thereby limiting DNA accessibility to cellular machineries that rely on DNA as a template. This histone protein, like others, holds a pivotal position in key cellular functions such as transcription regulation, DNA repair, DNA replication, and the maintenance of chromosomal stability. The intricate control of DNA accessibility involves a sophisticated system of post-translational modifications known as the histone code, along with dynamic nucleosome remodeling. The nucleosome itself is a histone octamer comprising two molecules each of H2A, H2B, H3, and H4, assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. This octamer efficiently wraps approximately 147 base pairs of DNA, underscoring its crucial role in organizing chromatin structure and facilitating essential genomic processes.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q7L7L0 (S2-K130)

Gene ID

92815  [NCBI]

Molecular Construction
N-term
HIST3H2A (S2-K130)
Accession # Q7L7L0
C-term
Synonyms
Histone H2A type 3; H2AW; HIST3H2A
AA Sequence

SGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Molecular Weight

15-20 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

HIST3H2A Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HIST3H2A Protein, Human
Cat. No.:
HY-P76382
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