HLA-E*0103 Complex Tetramer Protein, Human (HEK293, His-Avi)

The HLA-E*0103 complex is a nonclassical major histocompatibility class Ib molecule that is critical for immune self-non-self discrimination. It selectively binds the VL9 peptide from classical MHC class Ia molecules, forming a complex with B2M. HLA-E*0103 Complex Tetramer Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-E*0103 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-Avi, C-His labeled tag and VMAPRTLVL peptide. HLA-E*0103 Complex Tetramer Protein, Human (HEK293, His-Avi), has molecular weight of 260-265 kDa.

HLA-E*0103 Complex, a non-classical major histocompatibility class Ib molecule, plays a pivotal role in immune self-nonself discrimination. This molecule forms a complex with B2M/beta-2-microglobulin and selectively binds nonamer self-peptides derived from the signal sequence of classical MHC class Ia molecules, specifically VL9 peptides (VMAPRT[V/L][L/V/I/F]L). The peptide-bound HLA-E-B2M heterotrimeric complex serves as a ligand for the inhibitory receptor KLRD1-KLRC1 on natural killer (NK) cells, enabling these cells to monitor the expression of other MHC class I molecules in healthy cells and tolerate self. During cellular stress, HLA-E*0103 preferentially binds signal sequence-derived peptides from stress-induced chaperones, resulting in impaired protection from NK cells. Moreover, it binds signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules, acting as a ligand for the NK cell activating receptor KLRD1-KLRC2, potentially contributing to adaptive NK cell functions and maternal-fetal tolerance during pregnancy. Additionally, HLA-E*0103 can bind and present pathogen-derived peptides to alpha-beta T cell receptors on unconventional CD8-positive cytotoxic T cells, triggering an antimicrobial immune response. This molecule also presents specific peptides from viruses like human cytomegalovirus, facilitating immune tolerance to infected cells.

Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200).

Human

HEK293

C-Avi;C-His

P13747 (G22-T302)&P61769 (I21-M119)&VMAPRTLVL

3133  [NCBI]&567  [NCBI]

260-265 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.