HLA-G Complex Tetramer Protein, Human (Biotinylated, HEK293, His-Avi)

HLA-G, a non-classical major histocompatibility class Ib molecule, crucially regulates immunity at the maternal-fetal interface. Partnered with B2M, it forms a complex selectively binding self-peptides, fostering maternal-fetal tolerance through interactions with KIR2DL4, LILRB1, and LILRB2 receptors. Engagement triggers NK cell senescence, induces regulatory T cell differentiation, and balances tolerance and antiviral immunity. HLA-G actively contributes to immune modulation at the maternal-fetal interface, showcasing its multifaceted role in promoting fetal development while maintaining immune balance. HLA-G Complex Tetramer Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-G Protein and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-Avi, C-His labeled tag and RIIPRHLQL peptide. HLA-G Complex Tetramer Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 260-265 kDa.

HLA-G, a non-classical major histocompatibility class Ib molecule, plays a crucial role in immune regulation at the maternal-fetal interface. In association with B2M/beta-2 microglobulin, it forms a complex that selectively binds a limited repertoire of nonamer self-peptides derived from intracellular proteins, including histones and ribosomal proteins. This peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1, and LILRB2 receptors on uterine immune cells, fostering fetal development while maintaining maternal-fetal tolerance. Interactions with KIR2DL4 and LILRB1 receptors trigger NK cell senescence-associated secretory phenotype, promoting vascular remodeling and fetal growth during early pregnancy. Moreover, HLA-G's engagement with LILRB2 induces the differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, actively contributing to the maintenance of maternal-fetal tolerance. Additionally, HLA-G may play a role in balancing tolerance and antiviral immunity by modulating the effector functions of NK cells, CD8+ T cells, and B cells. Furthermore, it negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity, highlighting its multifaceted role in immune regulation at the maternal-fetal interface.

1. Human LILRB2 captured on CM5 Chip via Protein A can bind Biotinylated Human HLA-G Complex Tetramer with an affinity constant of 4.38 nM as determined in SPR assay (Biacore T200).
2. Serial dilutions of Anti-LILRB2 Antibody were added into Biotinylated HLA-G Complex Tetramer, His Tag: Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC₅₀) is 0.3 μg/mL.

Human

HEK293

C-Avi;C-His

P17693 (G25-T305)&P61769 (I21-M119)&RIIPRHLQL

3135  [NCBI]&567  [NCBI]

260-265 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.