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  4. HMBS/Porphobilinogen deaminase Protein, Human (HEK293, His, solution)

HMBS/Porphobilinogen deaminase Protein, Human (HEK293, His, solution)

Cat. No.: HY-P70269Y
COA Handling Instructions

HMBS (porphobilinogen deaminase) is key to heme biosynthesis, catalyzing the sequential polymerization of four porphobilinogen molecules to form hydroxymethylbiphenyl. The process begins with dipyrromethane cofactor assembly derived from porphobilinogen or preuroporphyrinogen and promoted by apoenzymes. HMBS/Porphobilinogen deaminase Protein, Human (HEK293, His, solution) is the recombinant human-derived HMBS/Porphobilinogen deaminase protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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10 μg $160 In-stock
50 μg $450 In-stock
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  • Biological Activity

  • Technical Parameters

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Description

HMBS (porphobilinogen deaminase) is key to heme biosynthesis, catalyzing the sequential polymerization of four porphobilinogen molecules to form hydroxymethylbiphenyl. The process begins with dipyrromethane cofactor assembly derived from porphobilinogen or preuroporphyrinogen and promoted by apoenzymes. HMBS/Porphobilinogen deaminase Protein, Human (HEK293, His, solution) is the recombinant human-derived HMBS/Porphobilinogen deaminase protein, expressed by HEK293 , with C-6*His labeled tag.

Background

HMBS, also known as Porphobilinogen deaminase, plays a crucial role in the heme biosynthetic pathway by catalyzing the sequential polymerization of four molecules of porphobilinogen, leading to the formation of hydroxymethylbilane, also referred to as preuroporphyrinogen. The catalytic process initiates with the assembly of the dipyrromethane cofactor, derived either from two molecules of porphobilinogen or from preuroporphyrinogen, by the apoenzyme. This covalently linked cofactor serves as a primer, facilitating the assembly of the tetrapyrrole product. In the final step of catalysis, the product preuroporphyrinogen is released, leaving the intact cofactor bound to the holodeaminase. This enzymatic activity is pivotal for the synthesis of heme, a crucial component with diverse biological functions.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P08397-1 (S2-H361)

Gene ID
Molecular Construction
N-term
HMBS (S2-H361)
Accession # P08397-1
6*His
C-term
Synonyms
rHuPorphobilinogen deaminase/HMBS, His; Porphobilinogen Deaminase; PBG-D; Hydroxymethylbilane Synthase; HMBS; Pre-Uroporphyrinogen Synthase; HMBS; PBGD; UPS
AA Sequence

SGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH

Molecular Weight

Approximately 47.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% Trehalose, 5% mannitol, 50% Glycerol, 0.1% Tween80, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

HMBS/Porphobilinogen deaminase Protein, Human (HEK293, His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HMBS/Porphobilinogen deaminase Protein, Human (HEK293, His, solution)
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HY-P70269Y
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