HNRPM Protein, Human (N-His)

The HNRNPM protein is a key pre-mRNA binding protein that exhibits strong affinity for poly(G) and poly(U) RNA homopolymers. It is central to the splicing process, complexly regulates RNA maturation, and serves as a receptor for carcinoembryonic antigen in Kupffer cells. HNRPM Protein, Human (N-His) is the recombinant human-derived HNRPM protein, expressed by E. coli , with N-10*His labeled tag. The total length of HNRPM Protein, Human (N-His) is 691 a.a., with molecular weight of ~75 kDa.

The HNRNPM protein emerges as a crucial pre-mRNA binding protein in vivo, exhibiting a strong affinity for poly(G) and poly(U) RNA homopolymers in vitro. Functionally implicated in splicing processes, HNRNPM plays a significant role in the intricate regulation of RNA maturation. Beyond its involvement in splicing, it acts as a receptor for carcinoembryonic antigen in Kupffer cells, potentially initiating a cascade of signaling events leading to the tyrosine phosphorylation of proteins and the induction of cytokines such as IL-1 alpha, IL-6, IL-10, and tumor necrosis factor alpha. Notably, HNRNPM is identified as a component of the spliceosome C complex, underscoring its association with fundamental RNA processing machinery. Moreover, the protein interacts with PPIA/CYPA, further expanding its network of molecular interactions and highlighting its multifaceted role in cellular processes.

Human

E. coli

N-10*His

P52272-2 (M1-A691)

4670

MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA

Approximately 75 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from sterile 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.