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HO-2/HMOX2 Protein, Human (His)

Cat. No.: HY-P79126
COA Handling Instructions

Through its catalytic activity, HO-2/HMOX2 protein promotes the oxidative cleavage of heme at the α-methylene bridge carbon, thereby releasing carbon monoxide (CO). This process simultaneously produces biliverdin IXalpha and releases the central heme iron chelate in the form of ferrous iron. HO-2/HMOX2 Protein, Human (His) is the recombinant human-derived HO-2/HMOX2 protein, expressed by E. coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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Description

Through its catalytic activity, HO-2/HMOX2 protein promotes the oxidative cleavage of heme at the α-methylene bridge carbon, thereby releasing carbon monoxide (CO). This process simultaneously produces biliverdin IXalpha and releases the central heme iron chelate in the form of ferrous iron. HO-2/HMOX2 Protein, Human (His) is the recombinant human-derived HO-2/HMOX2 protein, expressed by E. coli , with C-6*His labeled tag.

Background

Heme oxygenase-2 (HO-2), encoded by the HMOX2 gene, is a critical enzyme that catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, resulting in the release of carbon monoxide (CO) and the generation of biliverdin IXalpha. Simultaneously, the enzyme liberates the central heme iron chelate as ferrous iron. This reaction represents a pivotal step in heme catabolism and serves as a regulatory mechanism for maintaining cellular heme homeostasis. The release of carbon monoxide and biliverdin, along with the liberation of ferrous iron, underscores the multifunctional role of HO-2 in both gasotransmitter signaling and the recycling of iron. The enzymatic activity of HO-2 is essential for various physiological processes, including antioxidant defense, anti-inflammatory responses, and the regulation of vascular tone.

Biological Activity

Measured by its ability to oxidize hemin to biliverdin. The specific activity is >3.5 pmol/min/μg.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

P30519-1 (S2-L291)

Gene ID
Molecular Construction
N-term
HMOX2-1 (S2-L291)
Accession # P30519-1
6*His
C-term
Synonyms
HMOX2; HO-2; HO2; Heme Oxygenase 2
AA Sequence

SAEVETSEGVDESEKKNSGALEKENQMRMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLATTALYFTYSALEEEMERNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQCPKAAQKYVERIHYIGQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALDLNMKTKERIVEEANKAFEYNMQIFNELDQAGSTLARETLEDGFPVHDGKGDMRKCPFYAAEQDKGALEGSSCPFRTAMAVL

Molecular Weight

Approximately 31 & 35 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

HO-2/HMOX2 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HO-2/HMOX2 Protein, Human (His)
Cat. No.:
HY-P79126
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