HELPY Protein, Helicobacter pylori (His)

HpGT Protein, Helicobacter pylori is a glutathione-degrading enzyme that is a virulence factor in infection. Glutathione hydrolase proenzyme belongs to the gamma-glutamyltransferase family. pylorigamma-glutamyltranspeptidase is expressed as a 60-kDa inactive precursor that must undergo autocatalytic processing to generate a 40-kDa/20-kDa heterodimer with full gamma-glutamyl amide bond hydrolase activity^[1][2]. HELPY Protein, Helicobacter pylori (His) is the recombinant HpGT protein, expressed by E. coli , with N-6*His labeled tag. The total length of HELPY Protein, Helicobacter pylori (His) is 543 a.a., .

pylorigamma-glutamyltranspeptidase (HpGT) is expressed as a 60-kDa inactive precursor that must undergo autocatalytic processing to generate a 40-kDa/20-kDa heterodimer with full gamma-glutamyl amide bond hydrolase activity. The resultant N terminus, Thr-380, has been shown to be the catalytic nucleophile in both the autoprocessing and enzymatic reactions. HpGT is a member of the N-terminal nucleophile hydrolase superfamily. HpGT has also been shown to up-regulate COX-2 and epidermal growth factor-related peptides in human gastric mucosal cells and to induce apoptosis in human gastric epithelial cells^[1].
Helicobacter pylori γ-glutamyltranspeptidase (HpGT)  catalyzes the first step in glutathione degradation, removing glutamate from the tripeptide and its conjugates via a γ-glutamylenzyme intermediate. The γ-glutamyl group can be transferred to water (hydrolysis) or an amino acid or short peptide (transpeptidation)^[2].

Others

E. coli

N-6*His

O25743 (S25-F567, T380A)

/

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.