HSP60 Protein, Mouse (N-His)

The HSP60 protein, together with Hsp10, contributes to the import and assembly of mitochondrial proteins. It ensures correct folding and prevents incorrect folding under stressful conditions. HSP60 Protein, Mouse (N-His) is the recombinant mouse-derived HSP60, expressed by E. coli , with N-10*His labeled tag. The total length of HSP60 Protein, Mouse (N-His) is 572 a.a.,

The HSP60 protein plays a crucial role in mitochondrial protein import and macromolecular assembly. Along with Hsp10, it facilitates the proper folding of imported proteins and may also prevent misfolding while promoting the refolding and appropriate assembly of unfolded polypeptides generated during stressful conditions in the mitochondrial matrix. These chaperonins consist of heptameric rings formed by the large subunit Hsp60, which align in a back-to-back double ring configuration. Through a cyclic process, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by ATP binding and association with two heptameric rings of the co-chaperonin Hsp10. This leads to the encapsulation of the substrate protein within the inner cavity of Hsp60, allowing it to fold undisturbed by other cellular components for a certain period. Synchronized ATP hydrolysis in all Hsp60 subunits causes the dissociation of the chaperonin rings, releasing ADP and the properly folded substrate protein.

HSP60 has ATPase activity at the time of manufacture of 49.361 µM phosphate liberated/h/µg protein in a 200µL reaction at 37°C in the presence of 10 μL of 4 mM ATP using a Malachite Green assay.

Mouse

E. coli

N-10*His

P63038 (L2-F573)

15510

LRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIVTKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLIVEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.