Hyaluronidase-1/HYAL1 Protein, Human (HEK293, C-His)

Hyaluronidase-1/HYAL1 Protein is implicated in tumor progression, suggesting its role in cancer advancement. It also inhibits TGFB1-enhanced cell growth, revealing its regulatory function. HYAL1's dual impact on promoting tumor progression and inhibiting specific cellular processes underscores its complex involvement in cellular behavior and potential significance in tumorigenesis. Hyaluronidase-1/HYAL1 Protein, Human (HEK293, C-His) is the recombinant human-derived Hyaluronidase-1/HYAL1, expressed by HEK293 , with C-6*His labeled tag. The total length of Hyaluronidase-1/HYAL1 Protein, Human (HEK293, C-His) is 414 a.a.,

The Hyaluronidase-1/HYAL1 protein emerges as a potential contributor to tumor progression, suggesting its involvement in the intricate mechanisms that facilitate cancer advancement. Additionally, it exhibits the ability to impede TGFB1-enhanced cell growth, underscoring its regulatory function in restraining specific cellular processes. The dual nature of its impact on both promoting tumor progression and inhibiting TGFB1-enhanced cell growth underscores the complexity of HYAL1's involvement in cellular behavior and highlights its potential significance in the context of tumorigenesis.

Measured in a cell proliferation assay using DU145 cells. The ED₅₀ for this effect is 0.861 ng/mL, corresponding to a specific activity is 1.161×10⁶ units/mg.

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  Measured in a cell proliferation assay using DU145 cells. The ED₅₀ for this effect is 0.861 ng/mL, corresponding to a specific activity is 1.161×10⁶ units/mg.

Human

HEK293

C-6*His

Q12794-1 (F22-W435)

3373

FRGPLLPNRPFTTVWNANTQWCLERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASLIAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHPDWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYTGQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAAGDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQAIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLTPGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMW

Approximately 50-57 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.