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  3. Hydrolases (EC 3)
  4. IdeS Protein, Streptococcus pyogenes (His)

IdeS Protein, Streptococcus pyogenes (His)

Cat. No.: HY-P74867
COA Handling Instructions

IdeS Protein is a highly specific IgG endopeptidase evolved from Streptococcus pyogenes, which can degrade IgG and participate in the immune response.IdeS Protein inhibits the function of certain neutrophil effectors, namely the production of reactive oxygen species (ROS), independently of IgG endopeptidase activity. IdeS Protein, Streptococcus pyogenes (His) is the recombinant IdeS protein, expressed by E.coli , with N-10*His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $67 In-stock
10 μg $115 In-stock
50 μg $320 In-stock
100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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Description

IdeS Protein is a highly specific IgG endopeptidase evolved from Streptococcus pyogenes, which can degrade IgG and participate in the immune response.IdeS Protein inhibits the function of certain neutrophil effectors, namely the production of reactive oxygen species (ROS), independently of IgG endopeptidase activity. IdeS Protein, Streptococcus pyogenes (His) is the recombinant IdeS protein, expressed by E.coli , with N-10*His labeled tag.

Background

IdeS is a highly specific IgG endopeptidase evolved from Streptococcus pyogenes, which can not only degrade IgG but also directly or indirectly inhibit the innate immune response, thus promoting the survival of streptococcus in the inflammatory environment. IdeS was found to be identical to Mac-1 in Streptococcus, a protein thought to inhibit phagocytosis by inhibiting the recognition of IgG and/or complement configurations by the Fc receptor (CD16). IdeS/Mac-1 can inhibit the function of certain neutrophil effectors, namely the production of reactive oxygen species (ROS), independently of IgG endopeptidase activity[1][2].

Biological Activity

One unit digests ≥ 95% of 1µg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.

Species

Others

Source

E. coli

Tag

N-10*His

Accession

F8V4V0 (D30-N341)

Gene ID

/

Molecular Construction
N-term
10*His
IdeS (D30-N341)
Accession # F8V4V0
C-term
Synonyms
Immunoglubulin-degrading enzyme; ideS
Molecular Weight

Approximately 36.2 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, 50 % Glycerol, pH 6.6.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

IdeS Protein, Streptococcus pyogenes (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IdeS Protein, Streptococcus pyogenes (His)
Cat. No.:
HY-P74867
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