IDH1 Protein, Human (HEK293, C-His)

The IDH1 protein catalyzes the oxidative decarboxylation of isocitrate to generate 2-oxoglutarate, which is utilized by enzymes such as phytoacyl-CoA dioxygenase. IDH1 Protein, Human (HEK293, C-His) is the recombinant human-derived IDH1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of IDH1 Protein, Human (HEK293, C-His) is 414 a.a., with molecular weight of ~48 kDa.

IDH1 protein is a key enzyme that catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), a vital step in cellular metabolism and a process required by other enzymes such as the phytanoyl-CoA dioxygenase. The enzymatic activity of IDH1 is crucial for the generation of NADPH, a critical cofactor involved in various biosynthesis pathways. Additionally, IDH1 may have a distinct role as a corneal epithelial crystallin, potentially contributing to the maintenance of corneal epithelial transparency. These multifaceted functions highlight the importance of IDH1 in fundamental cellular processes and suggest its involvement in maintaining specific tissue characteristics.

Human

HEK293

C-6*His

O75874 (M1-L414)

3417

MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL

Approximately 48 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.