IDO-1 Protein, Mouse (His)

IDO Protein, an enzyme, is involved in the metabolism of tryptophan. Dysregulation of IDO Protein has been linked to immune escape mechanisms and tumor progression. Targeting IDO Protein may provide potential therapeutic interventions in cancer by modulating tryptophan metabolism, enhancing immune responses, and inhibiting tumor growth. IDO-1 Protein, Mouse (His) is the recombinant mouse-derived IDO protein, expressed by E. coli , with N-6*His labeled tag.

The IDO protein serves as a key catalyst in initiating and regulating the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Its pivotal role extends beyond metabolism, encompassing active participation in peripheral immune tolerance to maintain homeostasis by averting autoimmunity and immunopathology arising from unbridled immune responses. Notably, a shortage of tryptophan impedes T lymphocyte division, while the accumulation of tryptophan catabolites induces T-cell apoptosis and fosters the differentiation of regulatory T-cells. Additionally, IDO functions as a potent suppressor of anti-tumor immunity and plays a crucial role in limiting the growth of intracellular pathogens by depriving them of tryptophan. Furthermore, IDO is integral to safeguarding the fetus from maternal immune rejection, highlighting its diverse contributions to immune regulation and cellular equilibrium.

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine that incubate at room temperature for 5 minutes. The specific activity is 2576.39 pmol/min/µg.

Mouse

E. coli

N-6*His

P28776 (M1-P407)

15930  [NCBI]

MALSKISPTEGSRRILEDHHIDEDVGFALPHPLVELPDAYSPWVLVARNLPVLIENGQLREEVEKLPTLSTDGLRGHRLQRLAHLALGYITMAYVWNRGDDDVRKVLPRNIAVPYCELSEKLGLPPILSYADCVLANWKKKDPNGPMTYENMDILFSFPGGDCDKGFFLVSLLVEIAASPAIKAIPTVSSAVERQDLKALEKALHDIATSLEKAKEIFKRMRDFVDPDTFFHVLRIYLSGWKCSSKLPEGLLYEGVWDTPKMFSGGSAGQSSIFQSLDVLLGIKHEAGKESPAEFLQEMREYMPPAHRNFLFFLESAPPVREFVISRHNEDLTKAYNECVNGLVSVRKFHLAIVDTYIMKPSKKKPTDGDKSEEPSNVESRGTGGTNPMTFLRSVKDTTEKALLSWP

40-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 or 20mM Tris-HCl, 500mM NaCl, 0.01% Tween 80, 1mM EDTA, 50% Glycerol,pH 8.0 or 20 mM NaAc, 150 mM NaCl, pH 5.5, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.