IFIH1 Protein, Human (His)

The IFIH1 protein is an innate immune receptor that detects viral nucleic acids and triggers an antiviral response. IFIH1 is critical for type I interferon induction and recognizes ligands such as mRNA lacking 2'-O-methylation and long double-stranded RNA. IFIH1 Protein, Human (His) is the recombinant human-derived IFIH1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IFIH1 Protein, Human (His) is 326 a.a., with molecular weight of ~41.5 kDa.

IFIH1 protein, as an innate immune receptor, functions as a cytoplasmic sensor for viral nucleic acids, playing a pivotal role in detecting viral infections and initiating a cascade of antiviral responses. It is essential for inducing type I interferons and pro-inflammatory cytokines in response to viral stimuli. IFIH1 recognizes ligands such as mRNA lacking 2'-O-methylation at their 5' cap and long double-stranded RNA (>1 kb). Upon ligand binding, it associates with mitochondria antiviral signaling protein (MAVS/IPS1), activating the IKK-related kinases TBK1 and IKBKE, which phosphorylate interferon regulatory factors (IRF3 and IRF7), leading to the transcription of antiviral immunological genes, including interferons IFN-alpha and IFN-beta. The protein is responsible for detecting various viruses, including members of the Picornaviridae family, such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), rhinovirus, and SARS-CoV-2. IFIH1 is also involved in antiviral signaling against viruses with a double-stranded DNA genome, like vaccinia virus. Furthermore, it contributes to innate immune signaling amplification by recognizing RNA metabolites generated during virus infection by ribonuclease L (RNase L). Additionally, IFIH1 may enhance natural killer cell function and participate in growth inhibition and apoptosis in various tumor cell lines.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9BYX4 (K700-D1025)

64135  [NCBI]

KLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED

Approximately 41.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.