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  3. Hydrolases (EC 3)
  4. IFIH1 Protein, Human (His)

IFIH1 Protein, Human (His)

Cat. No.: HY-P71641
COA Handling Instructions

The IFIH1 protein is an innate immune receptor that detects viral nucleic acids and triggers an antiviral response. IFIH1 is critical for type I interferon induction and recognizes ligands such as mRNA lacking 2'-O-methylation and long double-stranded RNA. IFIH1 Protein, Human (His) is the recombinant human-derived IFIH1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IFIH1 Protein, Human (His) is 326 a.a., with molecular weight of ~41.5 kDa.

For research use only. We do not sell to patients.

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Description

The IFIH1 protein is an innate immune receptor that detects viral nucleic acids and triggers an antiviral response. IFIH1 is critical for type I interferon induction and recognizes ligands such as mRNA lacking 2'-O-methylation and long double-stranded RNA. IFIH1 Protein, Human (His) is the recombinant human-derived IFIH1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IFIH1 Protein, Human (His) is 326 a.a., with molecular weight of ~41.5 kDa.

Background

IFIH1 protein, as an innate immune receptor, functions as a cytoplasmic sensor for viral nucleic acids, playing a pivotal role in detecting viral infections and initiating a cascade of antiviral responses. It is essential for inducing type I interferons and pro-inflammatory cytokines in response to viral stimuli. IFIH1 recognizes ligands such as mRNA lacking 2'-O-methylation at their 5' cap and long double-stranded RNA (>1 kb). Upon ligand binding, it associates with mitochondria antiviral signaling protein (MAVS/IPS1), activating the IKK-related kinases TBK1 and IKBKE, which phosphorylate interferon regulatory factors (IRF3 and IRF7), leading to the transcription of antiviral immunological genes, including interferons IFN-alpha and IFN-beta. The protein is responsible for detecting various viruses, including members of the Picornaviridae family, such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), rhinovirus, and SARS-CoV-2. IFIH1 is also involved in antiviral signaling against viruses with a double-stranded DNA genome, like vaccinia virus. Furthermore, it contributes to innate immune signaling amplification by recognizing RNA metabolites generated during virus infection by ribonuclease L (RNase L). Additionally, IFIH1 may enhance natural killer cell function and participate in growth inhibition and apoptosis in various tumor cell lines.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q9BYX4 (K700-D1025)

Gene ID
Molecular Construction
N-term
6*His
IFIH1 (K700-D1025)
Accession # Q9BYX4
C-term
Synonyms
CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140kDa; DEAD/H (Asp Glu Ala Asp/His) box polypeptide; DEAD/H box polypeptide; Helicard; Helicase with 2 CARD domains; Hlcd; MDA-5
AA Sequence

KLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED

Molecular Weight

Approximately 41.5 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IFIH1 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFIH1 Protein, Human (His)
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