IFN-alpha 10/IFNA10 Protein, Human (HEK293, Fc)

IFN-alpha 10 (IFNA10), belongs to type I interferon family, is produced by macrophages with antiviral activities^[1]. IFN-alpha 10/IFNA10 Protein, Human (HEK293, Fc) contains 166 a.a. (C24-D189), produced in HEK293 cells with a N-terminal hFc-tag.

IFN-alpha 10 (IFNA10; IFN-α10), belongs to the alpha/beta interferon (IFN) family, is produced by the macrophages with antiviral activities^[1]. Interferon (IFN) is originally identified as a substance ‘interfering’ with viral replication in vitro. IFN-α/β and related molecules are classified as type I IFNs, as for the other two types of type II IFN (IFN-γ) and type III IFNs (IFN-λ), respectively^[2].
Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Interferon alpha (IFNa) shows significant biological activity in various cancers, paticularly haematological malignancies such as hairy cell leukaemia and chronic myelogenous leukaemia^[3].
IFNα-10 involves in JAK/STAT signaling pathway with strong signaling effect (EC₅₀=0.3 nM), is identified as potent regulators that reduces both CTLA4 and FOXP3 without affecting cell viability. Therefore, regulatory T cells (Tregs) as the key cells regulating peripheral autoreactive T lymphocytes, IFNα-10 regulates Treg functional states and destabilises Treg^[4].

IFN-alpha 1 (1^-11-1^-6 M; 24-26 h) results signifcant reduction of both CTLA4 and FOXP3 gene expression in regulatory T cells (Tregs) without affecting cell viability^[4].

Human

HEK293

N-hFc

P01566 (C24-D189)

3446  [NCBI]

Approximately 50 kDa.

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Kumagai Y, et al. Alveolar macrophages are the primary interferon-alpha producer in pulmonary infection with RNA viruses. Immunity. 2007 Aug;27(2):240-52.  [Content Brief]

  [2]. Zhang SY, et al. Inborn errors of interferon (IFN)-mediated immunity in humans: insights into the respective roles of IFN-alpha/beta, IFN-gamma, and IFN-lambda in host defense. Immunol Rev. 2008 Dec;226:29-40.  [Content Brief]

  [3]. Raj NB, et al. Identification of a novel virus-responsive sequence in the promoter of murine interferon-alpha genes. J Biol Chem. 1991 Jun 15;266(17):11360-5.  [Content Brief]

  [4]. Ding M, et al. Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells. Sci Rep. 2021 Aug 18;11(1):16767.  [Content Brief]