Ig kappa Protein, Human (HEK293, His)

Ig kappa protein is the constant region of an immunoglobulin light chain and is part of a membrane-bound or secreted glycoprotein produced by B lymphocytes. Ig kappa Protein, Human (HEK293, His) is the recombinant human-derived Ig kappa protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Ig kappa Protein, Human (HEK293, His) is 106 a.a., with molecular weight of ~14 kDa.

The constant region of immunoglobulin light chains, exemplified by Ig kappa protein, plays a crucial role in the structure and function of antibodies, also known as immunoglobulins. Produced by B lymphocytes, these membrane-bound or secreted glycoproteins serve as receptors in the recognition phase of humoral immunity. Upon binding a specific antigen, membrane-bound immunoglobulins initiate the clonal expansion and differentiation of B lymphocytes into plasma cells that secrete immunoglobulins. Secreted immunoglobulins, in turn, orchestrate the effector phase of humoral immunity, leading to the elimination of bound antigens. The antigen binding site is shaped by the variable domain of one heavy chain, in conjunction with that of its associated light chain, resulting in each immunoglobulin having two antigen binding sites with remarkable affinity for a particular antigen. The variable domains undergo V-(D)-J rearrangement and subsequent somatic hypermutations, allowing affinity maturation following exposure to antigen and selection. Immunoglobulins are characterized by two identical heavy chains and two identical light chains, intricately linked by disulfide bonds.

Human

HEK293

C-6*His

P01834 (T2-C107)

3514

TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Approximately 14 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.