IGF-I R Protein, Rat (HEK293, His)

Igf1r Protein, a cell surface receptor, is involved in growth regulation and development. Dysregulation of Igf1r Protein has been linked to diseases like cancer and growth disorders. Targeting Igf1r Protein may offer potential therapeutic interventions in these conditions by modulating cell growth, inhibiting tumor progression, and managing growth-related disorders. IGF-I R Protein, Rat (HEK293, His) is the recombinant rat-derived IGF-I R protein, expressed by HEK293 , with C-His labeled tag.

IGF1R is a receptor protein that plays a crucial role in cell growth and survival control. It is involved in tumor transformation and the survival of malignant cells. When a ligand, such as insulin-like growth factor 1 (IGF1), binds to IGF1R, it activates the receptor kinase, leading to autophosphorylation of the receptor and phosphorylation of multiple signaling adapter proteins, including insulin-receptor substrates (IRS1/2), Shc, and 14-3-3 proteins.Phosphorylation of IRS proteins activates two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. Activation of the MAPK pathway results in increased cellular proliferation, while activation of the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the regulatory subunit of PI3K (PIK3R1), which then activates downstream substrates, including protein AKT/PKB. AKT phosphorylation enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGF1R by phosphorylating and inactivating BAD.In addition to the PI3K-AKT/PKB and Ras-MAPK pathways, IGF1R also signals through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins leads to phosphorylation and activation of signal transducers and activators of transcription (STAT) proteins. The activation of STAT3, in particular, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may contribute to the transforming activity of IGF1R.The IGF1R can also activate JNK kinases. However, IGF1 exerts inhibitory effects on JNK activation by phosphorylating and inhibiting MAP3K5/ASK1, which can directly associate with IGF1R. Additionally, when IGF1R forms a hybrid receptor with the insulin receptor (INSR), it can bind IGF1.Overall, IGF1R plays a critical role in regulating cell growth, survival, and transformation through multiple signaling pathways, including the PI3K-AKT/PKB, Ras-MAPK, JAK/STAT, and JNK pathways.

Measured by its binding ability in a functional ELISA. When Rat IGF-I R is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IGF-I(HY-P7018). The ED₅₀ for this effect is 24.9 ng/mL.

Rat

HEK293

C-10*His

P24062 (E31-H936)

25718  [NCBI]

EICGPGIDIRNDYQQLKRLENCTVIEGFLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTIDWSLILDAVSNNYIVGNKPPKECGDLCPGTLEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSVCGKRACTENNECCHPECLGSCHTPDDNTTCVACRHYYYKGVCVPACPPGTYRFEGWRCVDRDFCANIPNAESSDSDGFVIHDGECMQECPSGFIRNSTQSMYCIPCEGPCPKVCGDEEKKTKTIDSVTSAQMLQGCTILKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWNHRNLTVRSGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLRFTSTTTWKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKEGEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPTLPNGNLSYYIVRWQRQPQDGYLFRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGDKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERRRRDVLQVANTTMSSRSRNTTVADTYNITDPEEFETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVPAKTTYENFMH

45-43 kDa, 100-130 kDa&140-180 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.