IGFBP-1 Protein, Mouse (SUMO, His)

The IGFBP-1 protein is an insulin-like growth factor binding protein that extends the half-life of IGF and modulates its growth-promoting effects in cell cultures, thereby inhibiting or stimulating these responses. Crucially, it alters the interaction between IGF and its cell surface receptors and promotes cell migration. IGFBP-1 Protein, Mouse (SUMO, His) is the recombinant mouse-derived IGFBP-1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of IGFBP-1 Protein, Mouse (SUMO, His) is 247 a.a., with molecular weight of ~43 kDa.

IGFBP-1, an insulin-like growth factor-binding protein, contributes to the regulation of IGFs by extending their half-life and modulating their growth-promoting effects on cell culture, either inhibiting or stimulating these effects. This protein plays a crucial role in altering the interaction between IGFs and their cell surface receptors. Additionally, IGFBP-1 demonstrates the ability to promote cell migration, indicating its involvement in cellular processes beyond growth regulation. Notably, it exhibits equal binding affinity for both IGF1 and IGF2, highlighting its versatile role in modulating the biological activities of insulin-like growth factors.

Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 1.317 µg/mL, corresponding to a specific activity is 759.3 U/mg.

Mouse

E. coli

N-6*His;N-SUMO

P47876 (A26-N272)

16006  [NCBI]

APQPWHCAPCTAERLGLCPPVPASCPEISRPAGCGCCPTCALPMGAACGVATARCAQGLSCRALPGEPRPLHALTRGQGACVPEPAAPATSTLFSSQHEEAKAAVVSADELSESPEMTEEQLLDSFHLMAPSREDQPILWNAISTYSSMRAREIADLKKWKEPCQRELYKVLERLAAAQQKAGDEIYKFYLPNCNKNGFYHSKQCETSLDGEAGLCWCVYPWSGKKIPGSLETRGDPNCHQYFNVHN

Approximately 43 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl,0.5 M NaCI, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.