IGFBP-2 Protein, Mouse (HEK293, His)

IGFBP-2 protein, a member of the IGF-binding protein family, inhibits IGF-mediated growth and developmental rates. It can extend the half-life of IGF and exhibit dual functions by inhibiting or stimulating the growth-promoting effect of IGF in cell culture. IGFBP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-2 Protein, Mouse (HEK293, His) is 271 a.a., with molecular weight of ~32-40 kDa.

IGFBP-2 protein acts as an inhibitor, specifically targeting IGF-mediated growth and developmental rates. As a member of the IGF-binding protein family, IGFBP-2 prolongs the half-life of IGFs and exhibits dual functionality, either inhibiting or stimulating the growth-promoting effects of IGFs in cell culture. This modulation occurs through the alteration of the interaction between IGFs and their cell surface receptors. Notably, IGFBP-2 demonstrates a higher binding affinity for IGF2 compared to IGF1, underscoring its role in finely regulating the intricate balance of IGF signaling pathways.

Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.2342 µg/mL in the presence of 30 ng/mL IGF-I, corresponding to a specific activity is 4.269×10³ units/mg.

Mouse

HEK293

C-His

P47877 (E35-Q305)

16008  [NCBI]

Approximately 32-40 kDa

Greater than 95% as determined by Tris-Bis PAGE

Lyophilized powder

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.