IGFBP-4 Protein, Mouse (HEK293, His)

IGFBP-4 protein modulates IGFs' activity and can either inhibit or stimulate their growth-promoting effects. It alters the interaction between IGFs and their receptors, with a preference for binding to IGF2. This highlights the nuanced role of IGFBP-4 in fine-tuning IGF-mediated cellular responses. IGFBP-4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-4 protein, expressed by HEK293 , with C-His labeled tag.

IGFBP-4 protein plays a pivotal role in modulating the activity of insulin-like growth factors (IGFs) by extending their half-life. Demonstrating a dual regulatory influence in cell culture, IGFBP-4 can either inhibit or stimulate the growth-promoting effects of IGFs, underscoring its versatile impact on cellular processes. Furthermore, IGFBP-4 is involved in altering the interaction between IGFs and their cell surface receptors. Notably, it exhibits a preference for binding to IGF2 over IGF1, indicating a specific affinity for particular IGF isoforms. This selective binding profile emphasizes the nuanced nature of IGFBP-4's role in fine-tuning the signaling pathways associated with IGF-mediated cellular responses.

Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. 1988, Cancer Research 48: 4083.) . The ED₅₀ for this effect is typically 0.1-0.6 μg/mL.

Mouse

HEK293

C-His

P47879 (D22-E254)

16010  [NCBI]

MLPFGLVAALLLAAGPRPSLGDEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGMRCYPPRGVEKPLRTLMHGQGVCTELSEIEAIQESLQTSDKDESEHPNNSFNPCSAHDHRCLQKHMAKIRDRSKMKIVGTPREEPRPVPQGSCQSELHRALERLAASQSRTHEDLFIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFQE

Approximately 38 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.