IGFBP-5 Protein, Cynomolgus (HEK293, His)

IGFBP-5 protein modulates IGFs, extending their life span and regulating their growth effects. It inhibits or stimulates IGFs, showing its dual function. By altering IGFs' interaction with cell receptors, IGFBP-5 fine-tunes growth-associated signaling pathways, governing cellular responses. This interplay reveals IGFBP-5's multifaceted role in cellular growth and proliferation. IGFBP-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IGFBP-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-5 Protein, Cynomolgus (HEK293, His) is 252 a.a., with molecular weight of ~35 kDa.

IGFBP-5 protein plays a pivotal role in modulating the activity of insulin-like growth factors (IGFs) by extending their half-life. Within cell culture, IGFBP-5 exhibits a dual regulatory function, demonstrating the capacity to either inhibit or stimulate the growth-promoting effects of IGFs. This dynamic influence underscores the intricacy of IGFBP-5's impact on cellular processes. Notably, IGFBP-5 achieves these regulatory effects by modifying the interaction between IGFs and their cell surface receptors, thereby finely regulating the signaling pathways associated with cellular growth and development. The nuanced interplay between IGFBP-5 and IGFs highlights the sophisticated control mechanisms that govern cellular responses to growth factors, shedding light on the multifaceted role of IGFBP-5 in orchestrating cellular growth and proliferation.

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ for this effect is 1.063 µg/mL in the presence of 14 ng/mL rhIGF-I, corresponding to a specific activity is 940.734 U/mg.

Cynomolgus

HEK293

C-His

G7PLE3 (L21-E272)

101926781  [NCBI]

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Approximately 35 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.