IGFBP-5 Protein, Canine (HEK293, Fc)

IGFBP-5 is only one of a family of six IGFBPs. IGFBP-5 is a 29 kDa phosphorylated and glycosylated protein, which is secreted by a wide variety of cell types during different growth conditions. IGFBP-5, the most conserved IGFBP, is the major protein secreted by skeletal muscles. IGFBP-5 Protein, Canine (HEK293, Fc) is the recombinant canine-derived IGFBP-5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IGFBP-5 Protein, Canine (HEK293, Fc) is 252 a.a., with molecular weight of ~40 & 43 & 45 & 65 KDa, respectively.

IGFBP-5 is only one of a family of six IGFBPs. IGFBP-5 is a 29 kDa phosphorylated and glycosylated protein, which is secreted by a wide variety of cell types during different growth conditions. IGFBP-5, the most conserved IGFBP, is the major protein secreted by skeletal muscles. IGFBP-5 enables fibronectin binding and is involved in regulation of insulin-like growth factor receptor signaling pathway^[1][2][3][4].

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ this effect is 1.468 µg/mL, corresponding to a specific activity is 681.199 U/mg.

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  Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ this effect is 1.468 µg/mL, corresponding to a specific activity is 681.199 U/mg.

Canine

HEK293

C-hFc

F1PEQ5 (L20-E271)

610316  [NCBI]

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQAKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Approximately 40&43&45&65 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.